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NAME:  Richard L. Gregory, Ph.D.
PHONE:  (317) 274-9949
FAX:  (317) 278-1411
E-MAIL:  rgregory@iupui.edu

 MUCOSAL IMMUNOBIOLOGY LABORATORY 

OFFICE ADDRESS: 

Indiana University School of Dentistry
Department of Oral Biology
Oral Health Research Institute
415 Lansing Street, Room 123
Indianapolis, Indiana  46202

  

POSITION/TITLE:

Professor of  Oral Biology and Preventive and Community Dentistry
Director, PhD. Dental Science Program
Director, Student Research Programs
School of Dentistry

Professor of Pathology and Laboratory Medicine, 
School of Medicine

TRAINING:
B.S., 1976: Medical Technology, Eastern Illinois University, Charleston
Ph.D., 1982: Microbiology and Immunology, Southern Illinois University, Carbondale
Postdoctoral, 1982-84: Mucosal Immunology, University of Alabama at Birmingham
Visiting Scientist, 1996: Dept. of Microbiology and Molecular Genetics, University of Vermont, Burlington 

RESEARCH INTERESTS:
Mucosal Immunobiology - To help understand the complex interactions in Streptococcus mutans-induced caries formation, we are defining the role cell surface proteins play in colonization and bacterial endocarditis. Mechanisms that may diminish colonization of S. mutans cells to enamel surfaces and cardiac tissue are being explored. Inhibitory factors being investigated include mucosal vaccines and neutralization of receptor sites.

In addition, mucosal immune responses to HIV antigens are being explored. Salivary and pulmonary responses to several HIV antigens are being studied using ELISA and immunoblotting. Antigens that healthy HIV seropositive subjects respond strongly to may be ideal candidates for mucosal vaccines. In addition, polyclonal B cell activation and the resultant hypergammaglobulinemia present in the systemic immune compartment are being investigated in the mucosal compartment.

A sensitive and specific in vitro caries model system has been developed in this laboratory and is being used to assess the ability of various anti-microbial agents and antibodies in prevention of S. mutans colonization of enamel surfaces and caries formation. In addition, the model is used to determine the relative cariogenicity of oral microorganisms. 

LINKS TO PATENTED TECHNOLOGIES:
 http://iurtc.iu.edu/ott/technol/9500/index.html
http://iurtc.iu.edu/ott/technol/0216/index.html

STUDENTS INVOLVED IN RESEARCH:
Diana Catt, Ph.D., Senior postdoctoral fellow; and Ph.D. students Jingping Ge, M.D., Zhiyun Chen, D.D.S., Janelle Renschler, D.V.M., and Vivian Wahaidi, D.D.S.

Click here to visit Dept. of Oral Biology research interests.

Dr. Gregory's lab (L to R):  J. Ge, D. Catt, J. Renschller, R. Gregory, Z. Chen, and V. Wahaidi

SELECT RECENT PUBLICATIONS (out of 278 total refereed publications and abstracts):  

Ge, J., Catt, D.M.,  and Gregory, R.L.   2004.  Streptococcus mutans surface α-enolase binds salivary MG2 and human plasminogen.  Infect. Immun. 72:6748-6752. 

Chang, J-C., Oshida, Y., Gregory, R.L., Andres, C.J., Barco, T.M. and Brown, D.  2003. Electrochemical study on microbiology-related corrosion of metallic materials.   Bio-Med. Mater. Eng. 13:281-295.

Dowling, J.S., Hockenberry, M., and Gregory, R.L.  2003.  Sense of humor, childhood cancer stressors, and outcomes of psychosocial adjustment, immune function, and infection.  J. Ped. Oncol.  Nursing 20:271-292.

Wood, K.L., Chaiyarit, P., Day, R.B., Wang, Y., Schnizlein-Bick, C.T., Gregory, R.L. and Twigg III, H.L. 2003.  Measurements of HIV viral loads from different levels of the respiratory tract.  Chest 124:536.542.

Sanders, B.J., Gregory, R.L., Moore, K. and Avery, D.R. 2002. Antibacterial and physical properties of resin modified glass-ionomers combined with chlorhexidine. J. Oral Rehabilitation 29:553-558.

Gonzalez-Cabezas, C., Li, Y., Gregory, R.L. and Stookey, G.K.  2002  Distribution of cariogenic bacteria in carious lesions around resin restorations.  Amer. J. Dent. 15:248-251.

Toro, M.J., Escalona, L., Chaiyarit, P., Warner, N.A., and Gregory, R.L.  2001.  Salivary immunoglobulin A antibodies to gp41 in human immunodeficiency virus-seropositive patients: lack of correlation with disease progression.  Oral Microbiol. Immunol.  16:188-192.

Gregory, R. L.  2001.  Evidence of modified immunogenicity of a mucosally administered antigen.  Clin. Diagn. Lab. Immunol.  8:540-544. 

Fontana, M., Buller, T.L., Dunipace, A.J., Stookey, G.K., and Gregory, R.L.  2000.  An in vitro microbial-caries model used to study the efficacy of antibodies to Streptococcus mutans surface proteins in preventing dental caries.  Clin. Diagn. Lab. Immunol.  7:49-54.

Escalona, L.A., Warner, N.A., Toro, M., and Gregory, R.L.  1999.  IgA antibodies to Escherichia coli in saliva from patients with HIV infection.  Acta. Odontologica Venezolana.  37:11-16.

Gonzalez-Cabezas, C., Li, Y., Gregory, R.L., and Stookey, G.K.  1999.  Distribution of three cariogenic bacteria in secondary carious lesions around amalgam restorations.  Caries Res.  33:357-365.

Chaiyarit, P., Kafrawy, A.H., Miles, D.A., Zunt, S.L., Van Dis, M.L., Gregory, R.L.  1999.  Oral lichen planus: an immunohistochemical study of heat shock proteins (HSPs) and cytokeratins (CKs) and a unifying hypothesis of pathogenesis. J. Oral Pathol. Med.  28:210-215.

Fontana, M., Dunipace, A.J., Stookey, G.K., and Gregory, R.L.  1999.  Intranasal immunization against dental caries with Streptococcus mutans enriched fimbrial preparation.  Clin. Diagn. Lab. Immunol.  6:405-409.

Ray, C.A., Gfell, L.E., Buller, T.L., and Gregory, R.L.  1999.  Interactions of Streptococcus mutans fimbria-associated surface proteins with salivary components.  Clin. Diagn. Lab. Immunol 6: 400-404.

Pascale, J.M., Shaw, M.M., Durant, P.J., Amador, A.A., Bartlett, M.S., Smith, J.W., Gregory, R.L., and  McLaughlin, G.L. 1999.   Intranasal immunization confers protection against murine Pneumocystis carnii infection.  Infect. Immun. 67: 805-809.

Gregory, R.L., Rahman, A.M.A., and Avery, D.R.  1998.  Effect of restorative treatment on mutans streptococci and IgA antibodies.  Ped. Dent.  20: 273-277.

Moore, M.A., Gregory, R.L., Switalski, L., Hakki, Z.W., Gfell, L.E., and Kowolik, M.J.  1998.  Differential activation of human neutrophils by Streptococcus mutans isolates from root surface lesions and caries free and caries active subjects.  Oral Microbiol. Immunol.  13:41-46.

Moore, M.A., Hakki, Z.W., Gregory, R.L., Gfell, L.E., Kim-Park, W.K., Kowolik, M.J.  1997.  Influence of heat inactivation of human serum on the opsonization of Streptococcus mutans.  In:  Phagocytes: Biology, Physiology, Pathology and Pharmacotherapeutics.  Annals of the New York Academy of Science,  832:383.393.
Gregory, R.L., Wallace, J.P., Gfell, L.E., Marks, J., and King, B.A.  1997.  Effect of exercise on milk immunoglobulin A levels.  Med. Sci. Sports Exerc.  29:1596-1601.
Pascale, J.M., Isaacs, M.D., Contreras, P., Gomez, B., Lozano, L., Austin, E., de Martin, M.C., Gregory, R.L., McLaughlin, G.L., and Amador, A.  1997.  Immunological markers of disease progression in patients infected with the human immunodeficiency virus.  Clin. Diagn Lab. Immunol. 4: 474-477.

Perrone, M., Gfell, L.E., Fontana, M., and Gregory, R.L.  1997.  Antigenic characterization of fimbriae preparations from Streptococcus mutans isolates from caries-free and caries susceptible subjects.  Clin. Diagn. Lab. Immunol. 4: 291-296.
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