Phone (317) 274-8822
Fax (317) 274-5425
Indiana University School of Dentistry
Oral Health Research Institute
415 Lansing St., Indianapolis, IN 46202-2876
Domenick T. Zero, DDS, MS, holds the position of Associate Dean for Research, Director of the Oral Health Research Institute and Professor and Chair of the Department of Preventive and Community Dentistry at Indiana University School of Dentistry. Dr. Zero previously held the position of Associate Director for Research and Associate Professor of Oral Sciences at Eastman Dental Center, Rochester, New York and Professor of Dentistry at the University of Rochester. After receiving his dental degree from Georgetown University, Washington, D.C., in 1975, Dr. Zero completed a general dentistry residency at the Eastman Dental Center and then a three-year research fellowship leading to a master's degree from the University of Rochester in 1979. As a principal investigator, he has received research grants from the National Institutes of Health and private industry. Dr. Zero has lectured extensively as an invited speaker throughout the country and abroad and is well recognized for his expertise in the field of Cariology and the use of in situ caries models. In 1996 he assumed the presidency of the Cariology Research Group of the International Association for Dental Research. From 1999 to 2001 he served as an elected member European Organization for Caries Research (ORCA) Advisory Board. He has also served as chair and vice-chair of the American Dental Association Council on Scientific Affairs, as a member of the Dental Products Panel of the Food and Drug Administration's Medical Devices Advisory Committee, and as a member of the Centers for Disease Control and Prevention's Expert Panel on School-based Dental Sealant Programs.
Studies on: the role of specific bacterial virulence factors in dental caries; improving the clinical efficacy of topical fluorides; fluoride distribution and clearance in the oral cavity; evaluation of the clinical efficacy of topical fluorides using in situ caries models; development and evaluation of new methods of early caries detection and caries lesion activity assessment; evaluation of etiology and prevention of dental erosion using in vitro and in situ models; Evaluating salivary diagnostic biomarkers.
Peer Reviewed Articles
Scaramucci T, Hara AT, Zero DT, Ferreira SS, Aokic IV, Sobral MAP. Development of an orange juice surrogate for the study of dental erosion. Braz Dent J 2011;22(6): 473-478.
Rethman MP, Beltrán-Aguilar ED, Billings RJ, Burne RA, Clark M, Donly KJ, Hujoel PP, Katz BP, Milgrom P, Sohn W, Stamm JW, Watson G, Wolff M, Wright JT, Zero D, Aravamudhan K, Frantsve-Hawley J, Meyer DM. Nonfluoride caries-preventive agents. Executive summary of evidence-based clinical recommendations. J Am Dent Assoc 2011;142;1065-1071.
Shellis RP, Ganss C, Ren Y, Zero D, Lussi A. Methodology and models in erosion research: discussion and conclusions. Caries Res 2011;45 (Suppl. 1):69–77.
Pereira AC, Eggertsson H, Gonzáles-Cabezas C, Zero DT, Eckert GJ, Mialhe FL. Quantitative light-induced fluorescence (QLF) in relation to other technologies and conventional methods for detecting occlusal caries in permanent teeth. Braz J Oral Sci 2011;10(1):27-32.
Lippert F, Lynch RJM, Eckert GJ, Kelly SA, Hara AT, Zero DT. In situ fluoride response of caries lesions with different mineral distributions at baseline. Caries Res 2011;45:47–55.
Martínez-Mier EA, Cury JA, Heilman J, Katz BP, Levy SM, Li Y, Maguire A, Margineda J, O’Mullane D, Phantumvanit P, Soto-Rojas AE, Stookey GK, Villa A, Wefel J, Whelton H, Whitford G, Zero DT, Zhang W, Zohouri V. Development of gold standard ion-selective electrode based methods for fluoride analysis. Caries Res 2011;45:3–12.
Zero DT, Ferreira Zandona A, Macapagal Vail M, Spolnik KJ. Dental Caries and Pulpal Disease. Dent Clin North Am 2011;55(1):29-46.
Fontana M, Jackson R, Eckert G, Swigonski N, Chin J, Ferreira Zandona A, Ando M, Stookey GK, Downs S, Zero DT. Identification of caries risk factors in toddlers. J Dent Res 2011;90(2):209-214.
Maggio B, Guibert RG, Mason SC, Karwal R, Rees GD, Kelly S, Zero DT. Evaluation of mouthrinse and dentifrice regimens in an in situ erosion remineralisation model. J Dent 2010;38(S3):S37–S44.
Engle K, Hara AT, Matis B, Eckert GJ, Zero DT. Erosion and abrasion of enamel and dentin associated with at-home bleaching: an in vitro study. J Am Dent Assoc 2010;141(5):546-51.
Hara AT, Zero DT. The caries environment: saliva, pellicle, diet, and hard tissue ultrastructure. Dent Clin North Am 2010;54(3):455-467.
Fontana M, Zero, DT, Gray, S, Beltran, ED. Techniques for tooth surface assessments in school-based sealant programs. J Am Dent Assoc 2010;141;854-860.
Ferreira Zandona A, Santiago E, Eckert G, Fontana M, Ando M, Zero DT. The use of ICDAS combined with QLF as a caries detection method. Caries Res 2010;44:317-322.
Zero DT, Creeth JE, Bosma ML, Butler A, Guibert RG, Karwal R, Lynch RJM, Martinez-Mier EA, González-Cabezas C, Kelly SA. The effect of brushing time and amount of dentifrice on fluoride delivery in vivo and enamel surface microhardness in situ. Caries Res 2010;44:90-100.
Ando M, Eckert GJ, Zero DT. Preliminary study to establish a relationship between tactile sensation with surface roughness. Caries Res 2010;44:24–28.
Steiner-Oliveira CC, Nobre-dos-Santos M, Zero DT, Eckert G, Hara AT. Effect of a pulsed CO2 laser and fluoride on the prevention of enamel and dentin erosion. Arch Oral Biol 2010;55:127-133.
Martínez-Mier EA, Soto-Rojas AE, Margineda J, Zero DT. Evaluation of the direct and diffusion methods for the determination of fluoride content in table salt. Community Dent Health 2009;26:204-210.
Pereira AC, Eggertsson H, Moustafa A, Zero DT, Eckert GJ, Mialhe FL. Evaluation of three radiographic methods for detecting occlusal caries lesions. Braz J Oral Sci 2009;8: 67-70.
Milgrom P, Zero DT, Tanzer JM. An Examination of the advances in science and technology of prevention of tooth decay in young children since the surgeon general's report on oral health. Acad Pediatr 2009;9:404–409.
Gooch BF, Griffin SO, Gray SK, Kohn WG, Rozier RG, Siegal M, Fontana M, Brunson D, Carter N, Curtis DK, Donly KJ, Haering H, Hill LF, Hinson HP, Kumar J, Lampiris L, Mallatt M, Meyer DM, Miller WR, Sanzi-Schaedel SM, Simonsen R, Truman BI, Zero DT. Preventing dental caries through school-based sealant programs: updated recommendations and reviews of evidence. J Am Dent Assoc 2009;40:1356-1365.
Hara AT, González-Cabezas C, Creeth J, Parmar M, Zero DT. Interplay Between Fluoride and Abrasivity of Dentifrices on Dental Erosion-Abrasion. J Dent 2009;37:781-785.
Zero DT, Fontana M, Martínez-Mier EA, Ferreira-Zandoná A, Ando M, González-Cabezas C, Bayne S. American Contributions to Scientific Advances in the Biology, Prevention, Diagnosis and Treatment of Dental Caries. J Am Dent Assoc 2009;140(Suppl 1):25S-34S.
Zero DT. Recaldent™ – Evidence for clinical activity. Adv Dent Res 2009;21:30-34.
Fontana M, Catt D, , Eckert GJ, Ofner S, Toro M, Gregory RL, Ferreira Zandona A, Eggertsson H, Jackson R, Zero D, Sissons C. Xylitol: effects on the acquisition of cariogenic species in infants. Pediatr Dent 2009;31:257-266.
Hara AT, Kelly SA, González-Cabezas C, Eckert GJ, Barlow AP, Mason SC, Zero DT. Influence of fluoride availability of dentifrices on eroded enamel remineralization in situ. Caries Res 2009;43:57–63.
Pereira AC, Eggertsson H, Martinez-Mier EA, Mialhe FL, Eckert GJ, Zero DT. Validity of caries detection on occlusal surfaces and treatment decisions based on results from multiple caries-detection methods. Eur J Oral Sci 2009;117:51–57.
Hara AT, González-Cabezas C, Creeth J, Zero DT. The effect of human saliva substitutes in an erosion-abrasion cycling model. Eur J Oral Sci 2008;116:552–556.
Zero DT. Are sugar substitutes also anticariogenic? J Am Dent Assoc 139 (suppl2):9S-10S, 2008.
Griffin S, Oong E, Kohn W, Vidakovic B, Gooch B, Bader J, Clarkson J, Fontana M, Meyer D, Rozier G, Weintraub J, Zero D. The effectiveness of sealants in managing carious lesions. J Dent Res 2008;87:169-174.
Hara AT, Karlinsey RL, Zero DT. Dentine remineralisation by simulated saliva formulations with different Ca and P(i) contents. Caries Res 2008;42:51-56.
Hara AT, Zero DT. Analysis of the erosive potential of calcium-containing acidic beverages. Eur J Oral Sci 2008;116:1-6.
Fontana M, Zero D. Bridging the gap in caries management between research and practice through education: The Indiana University experience. J Dent Educ 2007;71:579-591.
Totiam P, González-Cabezas C, Fontana MR, Zero DT. A new in vitro model to study the relationship of gap size and secondary caries. Caries Res 2007;41:467-473.
Lussi A, Zero D, Hellwig E. Erosive tooth wear: Diagnosis-risk factors-prevention. Am J Dent 2006;19:319-325.
Zandona AF, Zero DT. Diagnostic tools for early caries detection. J Am Dent Assoc 2006;137:1675-1684.
Zero DT, Hara AT, Kelly SA, González-Cabezas C, Eckert GJ, Barlow AP, Mason SC. Evaluation of a desensitizing test dentifrice using an in situ erosion remineralization model. J Clin Dent 2006;17:112-116.
Hara AT, Turssi CP, Ando M, González-Cabezas C, Zero DT, Rodrigues AL Jr, Serra MC, Cury JA. Influence of fluoride-releasing restorative material on root dentine secondary caries in situ. Caries Res 2006;40:435-439.
Hara AT, Ando M, González-Cabezas C, Cury JA, Serra MC, Zero DT. Protective effect of the pellicle against erosive challenges in situ. J Dent Res 2006;85:612-616.
Fontana M, Zero DT. Assessing your Patient’s Caries Risk. J Am Dent Assoc 2006;137:1231-1239.
Zero DT. Dentifrices, mouthwashes, remineralization/caries arrestment strategies. BMC Oral Health Jun 2006;15;6 Suppl 1:S9. http://www.biomedcentral.com/1472-6831/6/S1/S9
Ando M, Stookey GK, Zero DT. Ability of quantitative light-induced fluorescence (QLF) to assess the activity of white spot lesions during dehydration. Am J Dent 2006;19:15-18.
Zero DT, Lussi A. Erosion -- Chemical and biological factors of importance to the dental practitioner. Int Dent J 2005;55 (Suppl 4):285-291.
Zero D, Lussi A. Behavioral factors. In Dental Erosion: From Diagnosis to Therapy. Monogr Oral Sci 2006;20:100-105.
Hara A, Lussi A. Zero D. Biological factors. In Dental Erosion: From Diagnosis to Therapy. Monogr Oral Sci 2006;20:88-99.
Sá Roriz Fonteles C, Zero DT, Moss ME, Fu J. Fluoride concentrations in enamel and dentin of primary teeth after pre- and postnatal fluoride exposure. Caries Res 2005;39:505-508.
Martínez-Mier EA, Soto-Rojas AE, Buckley CM, Zero DT, Margineda J. Fluoride concentration of bottled water, tap water, and fluoridated salt from two communities in Mexico. Int Dent J 2005;55:93-99.
Fontana M, Zunt S, Eckert GJ, Zero D. A screening test for unstimulated salivary flow measurement. Oper Dent 2005;30:3-8.
Hara AT, Ando M, Cury JA, Serra MC, González-Cabezas C, Zero DT. Influence of the organic matrix on root dentine erosion by citric acid. Caries Res 2005;39:134-138.
Martínez-Mier EA, Soto-Rojas AE, Buckley CM, Margineda J, Stookey GK, Zero DT. [Evaluation of fluoride content in Mexican salt.] Salud Publica Mex 2004;46:197-198. (Spanish)
Zero D. Sugars – The Arch Criminal? Caries Res 2004;38:277-285.
Ando M, Eckert GJ, Stookey GK, Zero DT. Effect of imaging geometry on evaluating natural white-spot lesions using quantitative light-induced fluorescence. Caries Res 2004;38:39-44.
Zero DT, Zhang JZ, Harper DS, Wu M, Kelly S, Waskow J, Hoffman M. The remineralizing effect of an essential oil fluoride mouthrinse in an intraoral caries test. JADA 2004;135:231-237.
Lussi A, Jaeggi T, Zero D. Role of diet in the aetiology of dental erosion. Caries Res 2004;38(suppl 1):34-44.
Owens JD, Dowsett SA, Eckert GJ, Zero DT, Kowolik, MJ. Partial-mouth assessment of periodontal disease in an adult population of the United States. J Periodontol 2003;74:1206-1213.
Paes Leme AF, Machado Tabchoury CM, Zero DT, Cury JA. The effect of fluoride dentifrice and acidulated phosphate fluoride application on early artificial carious lesions. Am J Dent 2003;16:91-95.
Ando M, Schemehorn BR, Zero DT, Stookey GK. Influence of enamel thickness on quantification of mineral loss in enamel using laser-induced fluorescence. Caries Res 2003;37:24-28.
Zero D, Fontana M, Lennon ÁM. Clinical applications and outcomes of using indicators of risk in caries management. J Dent Educ 2001;65:1126-1132.
Hofstede MT, Ercoli C, Graser GN, Tallents, RH, Moss ME, Zero DT. The influence of metal surface finishing on porcelain porosity and bond strengths at the metal-ceramic interface. J Prosth Dent 2000;84:309-317.
Lussi A, Kohler N, Zero D, Schaffner M, Megert B. Comparison of the erosive potential of different beverages in primary and permanent teeth using an in vitro model. Eur J Oral Sci 2000;108:110-114.
Zero DT. Dental Caries Process. Dent Clin North Am 1999;43:635-664.
Zero DT. Application of clinical models in remineralization research. J Clin Dent 1999;10 (Spec Iss):74-85.
Handelman S, Zero DT. Dr. Basil Bibby: Early fluoride investigator and intellectual provocateur. J Dent Res 1997;76:1621-1624.
Zero DT. Etiology of dental erosion – extrinsic factors. Eur J Oral Sci 1996;104:162-177.
Moss ME, Zero DT. An overview of caries risk assessment and its potential utility. J Dent Educ 1995;59:932-940.
Zero DT. Introduction to the Conference on the Future Direction of Dental Postgraduate Education: A Tribute to Basil G. Bibby on his 90th Birthday. J Dent Educ 1995;59:546-547.
Zero DT. In situ caries models. Adv Dent Res 1995;9:214-230.
McCormack SM, Maran CMD, Scott-Anne KM, Zero DT. A comparison of cell sediment and surface grown "test plaque" using scanning electron microscopy. Scanning Microscopy 1995;9:207-214.
Saunders RH, Davilla CE, Hayes AL, Fu J, Zero DT. The effectiveness of sponge-type intraoral applicators in applying topical fluorides in nursing home elderly. Special Care 1994;14: 224-228.
Harding AM, Zero DT, Featherstone JDB, McCormack SM, Shields CP, Proskin HM. Calcium fluoride formation on sound enamel using fluoride solutions with and without lactate. Caries Res 1994;28:1-8.
Aguirre-Zero O, Zero DT, Proskin HM. Effect of chewing xylitol chewing gum on salivary flow rate and the acidogenic potential of dental plaque. Caries Res 1993;27:55-59.
Zero DT, Raubertas R F, Fu J, Pedersen AM, Hayes A L, Featherstone JDB. Fluoride concentrations in plaque, whole saliva and ductal saliva after application of home-use topical fluorides. J Dent Res 1992;71:1768-1775.
Zero DT, Raubertas RF, Pedersen AM, Fu J, Hayes A, Featherstone JDB. Studies of fluoride retention by the oral soft tissues after application of home-use topical fluorides. J Dent Res. 1992;71:1546-1552.
Featherstone JDB, Zero DT. An in situ model for simultaneous assessment of inhibition of demineralization and enhancement of remineralization. J Dent Res 1992;71 (Spec Iss):804-810.
Zero DT, Fu J, Anne KM, Cassata S, McCormack SM, Gwinner LM. An improved intraoral enamel demineralization test model for the study of dental caries. J Dent Res 1992;71 (Spec Iss):871-878.
Meyerowitz C, Featherstone JDB, Billings RJ, Eisenberg AD, Fu J, Shariati M, Zero DT. Evaluation of 0.05 percent sodium fluoride mouth rinse in radiation-induced hyposalivation using an intra-oral model. J Dent Res 1991;70:894-898.
Zero DT, Rahbek I, Fu J, Proskin HM, Featherstone JDB. Comparison of the iodide permeability test, the surface microhardness test and mineral dissolution of bovine enamel following acid challenge. Caries Res 1990;24:181-188.
Zero DT, Fu J, Espeland MA, Featherstone JDB. Comparison of fluoride concentrations in unstimulated whole saliva following the use of a fluoride dentifrice and a fluoride rinse. J Dent Res 1988;67:1257-1262.
Bibby BG, Mundorff SA, Zero DT, Almekinder KJ. Oral food clearance and the pH of plaque and saliva. J Am Dent Assoc 1986;112:333-337.
Zero DT, Van Houte J, Russo J. Enamel demineralization by acid produced from endogenous substrate in oral streptococci. Arch Oral Biol 1986;31:229-234.
Zero DT, Van Houte J, Russo J. The intraoral effect on enamel demineralization of extracellular matrix material synthesized from sucrose by Streptococcus mutans. J Dent Res 1986;65:918-923.
Zero DT, Curzon MEJ, Zander HA. Physical and chemical effects of toothpastes on dental enamel. J Dent Res 1982;61:451-455.
Bibby BG, Huang CT, Zero D, Mundorff SA, Little MF. Protective effect of milk against in vitro caries. J Dent Res 1980;59:1565-1570.
Longbottom C, Ekstrand K, Zero D. Traditional Preventive Treatment Options. In Detection, Assessment, Diagnosis and Monitoring of Caries. Ed. Pitts NB, Monogr Oral Sci. Basel, Karger, 2009, vol 21, pp 149–155. (DOI: 10.1159/000224219)
Longbottom C, Ekstrand K, Zero D, Kambara M. Novel preventive treatment options. In Detection, Assessment, Diagnosis and Monitoring of Caries. Ed. Pitts NB, Monogr Oral Sci. Basel, Karger, 2009, vol 21, pp 156–163. (DOI: 10.1159/000224220)
Ekstrand KR, Zero DT, Martignon S, Pitts NB. Lesion activity assessment. In Detection, Assessment, Diagnosis and Monitoring of Caries. Ed. Pitts NB, Monogr Oral Sci. Basel, Karger, 2009, vol 21, pp 63–90. (DOI: 10.1159/000224213) Monogr Oral Sci 21:63-90, 2009. Review.
Zero DT, Moynihan P, Lingström P, Birkhed D. The role of dietary control. In Dental Caries: The Disease and its Clinical Management. Eds. Fejerskov O and Kidd E, Blackwell Munksgaard, 2008, pp. 329-352.
Ismail AI, Banting D, Eggertsson H, Ekstrand KR, Ferreira-Zandoná A, Longbottom C, Pitts NB, Reich E, Ricketts D, Selwitz R, Sohn W, Topping GV, Zero D. Rationale and evidence for the International Caries Detection and Assessment System (ICDAS II). In: Clinical Models Workshop: Remin-Demin, Precavitation, Caries. Proceedings of the 7th Indiana Conference, Stookey GK, editor, Indianapolis, Indiana University School of Dentistry, 2005, pp. 161-221.
Eggertsson H, Ferreira-Zandoná AG, Ando M, González-Cabezas C, Fontana M, Martínez-Mier EA, Waskow-Chin JR, Jackson RD, Eckert GJ, Stookey GK, Zero DT: Reproducibility of in vitro and clinical examinations with QLF (Quantitative Light-induced Fluorescence). Developing guidelines for imaging and analyzing QLF- images, and a process for training examiners. In: Stookey GK, ed. Early detection of dental caries III: proceedings of the 6th annual Indiana Conference. Indianapolis, Indiana: Indiana University School of Dentistry 2003:213-228.
Zero DT, González-Cabezas C, Fontana M. Oral factors to consider that influence restorative treatment. In The Changing Practice of Restorative Dentistry. Ed. S Duke, Indianapolis, Indiana University School of Dentistry, 2002, pp. 87-103.
Zero D, Mol A, Sá Roriz C, Spoon M, Jacobs A, Keem S, Elbaum M. Caries detection using digital imaging fiber-optic transillumination (DIFOTI™) - A Preliminary Evaluation. In Early Detection of Dental Caries II. Ed. G Stookey, Indianapolis, Indiana University School of Dentistry, 2000, pp. 169-183.
Zero DT, Lussi A. Etiology of enamel erosion - intrinsic and extrinsic factors. (chapter) in Tooth Wear and Sensitivity. Ed. M. Addy, G. Embery, M. Edgar, R. Orchardson, Martin Dunitz, Ltd, 2000, pp. 121-139.
Berkowitz RJ, Zero D, Edelstein B. Contributor: "Dental Caries" (chapter) in Twenty Common Problems in Preventive Health Care. Ed. D. Campos-Outcalt, McGraw-Hill, 2000 pp. 65-78.
Lussi A, Zero D. External risk factors in childhood dental erosion. Nederlandse Vereniging voor Kindertandheelkunde. Bohn Stafleu Van Loghum: 7-12, 1999.
Zero DT. Adaptations in dental plaque. In: "Cariology for the Nineties". Bowen W.H. and Tabak, L., eds. University of Rochester Press, Rochester, pp. 333-350, 1993.
Featherstone JDB, Zero DT. Laboratory and human studies to elucidate the mechanism of action of fluoride-containing dentifrices. In: Clinical and Biological Aspects of Dentifrices, Emberg, G. and Rolla G., editors. pp 41-50 Oxford University Press, 1992.
Curzon MEJ, Zero DT, Treasure PE, Eisenberg AD, Featherstone JDB. Evaluation of a sterilizable artificial mouth to study plaque pH and enamel demineralization. Foods, Nutrition and Dental Health Conference, Vol. IV, J. Hefferen, W. Ayer, H. Koehler, C. McEmery, eds. Chicago, American Dental Association Publishers, 1984, pp. 7-14.
Bibby BG, Huang CT, Brown JP, Zero DT, and Curzon MEJ. pH measurements of in vitro dental plaque. Foods, Nutrition and Dental Health, Proceeding of the Foods, Nutrition and Dental Health Conference, Vol. III, J. Hefferren and H. Koehler, eds. Chicago, Pathotox Publishers, 1981, pp. 93-104.