Ph.D. in Biochemistry and Molecular Biology, The University of Melbourne and St. Vincent’s Institute of Medical Research, Victoria, Australia.

Postdoctoral Fellowship, Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Associate Research Scientist, Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, CT, USA.

The maintenance of bone mass in adult life depends upon the coordinated activities of both the osteoblasts, which make new bone, and the osteoclasts, the cells responsible for bone degradation. In skeletal diseases such as osteoporosis, Paget’s disease and hypercalcemia of malignancy, unregulated bone resorption by osteoclasts results in low bone mass, skeletal fragility and increased risk of bone fracture. The metabolic bone diseases and periodontitis also result in alveolar bone loss, tooth mobility and ultimately, tooth loss.

Osteoclast adhesion and migration along the bone surface involves remodeling of its cytoskeleton and the assembly, organization and disassembly of specialized adhesion structures named podosomes. My laboratory is focused on identifying and characterizing key intracellular signaling proteins and protein complexes that are involved in regulating the attachment, migration and bone resorbing activity of osteoclasts. To this end, we are currently using a number of transgenic and knockout mice to investigate the role of specific tyrosine kinases, actin-regulatory proteins and GTP-binding/hydrolyzing proteins in regulating osteoclast function.

A. Bruzzaniti, L. Neff, A. Sandoval, W.C. Horne and R. Baron. Src regulates its own binding to Pyk2 in podosomes by promoting the dynamin GTPase-dependent decrease in Pyk2 phosphorylation. J. Biol. Chem. (2008)

W. Horne, A. Bruzzaniti, C. Itzstein, A. Sanjay and R. Baron. The roles of c-Cbl and Cbl-b in bone: Multiple functions of Cbl proteins in a complex tissue, In "Cbl proteins", Nova Science Publishers, in press (2008).

H. Gil-Henn, O. Destaing, N.A. Sims, K. Aoki, N. Alles, L. Neff, A. Sanjay, A. Bruzzaniti, P. De Camilli, R. Baron, and J. Schlessinger. Defective Pyk2-mediated Podosome Organization in Osteoclasts Leads to Osteopetrosis in Pyk2-/- mice. J. Cell Biol., 178:1053-64 (2007)

A. Bruzzaniti and R. Baron. Molecular Regulation of Osteoclast Activity. Reviews in Endocrine and Metabolic Disorders, 7:123-39 (2006)

W.C. Horne, A. Sanjay, A. Bruzzaniti and R. Baron. The Role(s) of Src Family Kinases and Cbl Proteins in the Regulation of Osteoclasts. Immunological Reviews, 208:106-125 (2005)

A. Bruzzaniti. The Biology of Osteoporosis: Examination of Key Proteins Involved in Bone Loss. Innovations in Women’s Health, 4:7 (2005)

A. Bruzzaniti, L. Neff, A. Sanjay, W.C. Horne, P. De Camilli and R. Baron. Dynamin Forms a Src-Kinase-sensitive Complex with Cbl and Regulates Podosomes and Osteoclast Activity. Mol. Biol. Cell, 16:3301-3313 (2005)

A. Bruzzaniti and R.E. Mains. Enzymatic Activity of Soluble and Membrane Tethered Peptide Prohormone Convertase 1, Peptides, 23:863-875 (2002)

R. El Meskini, L. Jin, R. Marx, A. Bruzzaniti, J. Lee, R. Emeson and R. Mains. A Signal Sequence is Sufficient for Green Fluorescent Protein to be Routed to Regulated Secretory Granules. Endocrinology, 142:864-73 (2001)

A. Bruzzaniti, R. Marx and R.E. Mains. Activation and Processing of Membrane-tethered Prohormone Convertases 1 and 2. J. Biol. Chem., 274:24703-247013 (1999)

A. Bruzzaniti, K. Goodge, K., P. Jay, S.A. Taviaux,, M.H.C. Lam, P. Berta, T.J. Martin, J.M. Moseley and M.T. Gillespie. PC8, a new Member of the Convertase Family. Biochemical J., 314:727-731 (1996)